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Multiphoton fluorescence microscopy

Multiphoton microscopy is a fluorescence imgaging technique used frequently in bio-medical applications for imaging of living-cells. It is a variation of two-photon microscopy. Usually MIR/IR light is used to excite fluorescent dyes in cells, in which case, two photons are absorbed, and one photon of a shorter wavelength is emitted, causing fluorescence.

The usage of MIR/IR light minimises scattering from the cell tissue examined. Two/Multiple-photon absorption also allows background signal suppression techniques. These together increases penetration depth of microscopy. Compare to conventional confocal microscopy, it is capable of deeper penetration, more efficient detection and less phototoxicity. 


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